Magnetic Particle-Based Sandwich Sensor with DNA-Modified Carbon Nanotubes as Recognition Elements for Detection of DNA Hybridization

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• 作者：Po Hu ; Cheng Zhi Huang ; Yuan Fang Li ; Jian Ling ; Yu Ling Liu ; Liang Run Fei ; Jian Ping Xie
• 刊名：Analytical Chemistry
• 出版年：2008
• 出版时间：March 1, 2008
• 年：2008
• 卷：80
• 期：5
• 页码：1819 - 1823
• 全文大小：241K
• 年卷期：v.80,no.5(March 1, 2008)
• ISSN：1520-6882

文摘

In this contribution, we design a visual sensor for DNAhybridization with DNA probe-modified magnetic particles(MPs) and multiwalled carbon nanotubes (MWNTs) without involving a visual recognition element such as fluorescent/chemiluminescent reagents. It was found thatDNA probe-modified MWNTs, which could be dispersedin aqueous medium and have strong light scatteringsignals under the excitation of a light beam in the UV-vis region, could connect with DNA probe-modified MPstogether in the presence of perfectly complementary targetDNA and form a sandwich structure. In a magnetic field,the formed MP-MWNT species can easily be removedfrom the solution, resulting in a decrease of light scattering signals. Thus, a magnetic particle-based sandwichsensor could be developed to detect DNA hybridizationby measuring the light scattering signals with DNA-modified MWNTs as recognition elements. Experimentsshowed that the DNA-modified MPs sensor could bereused at least 17 times and was stable for more than 6months.