Constructing de Novo H2O2 Signaling via Induced Protein Proximity

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• 作者：Guihua Zeng ; Roushu Zhang ; Weimin Xuan ; Wei Wang ; Fu-Sen Liang
• 刊名：ACS Chemical Biology
• 出版年：2015
• 出版时间：June 19, 2015
• 年：2015
• 卷：10
• 期：6
• 页码：1404-1410
• 全文大小：376K
• ISSN：1554-8937

文摘

A new chemical strategy has been developed to generate de novo signaling pathways that link a signaling molecule, H₂O₂, to different downstream cellular events in mammalian cells. This approach combines the reactivity-based H₂O₂ sensing with the chemically induced protein proximity technology. By chemically modifying abscisic acid with an H₂O₂-sensitive boronate ester probe, novel H₂O₂ signaling pathways can be engineered to induce transcription, protein translocation and membrane ruffle formation upon exogenous or endogenous H₂O₂ stimulation. This strategy has also been successfully applied to gibberellic acid, which provides the potential to build signaling networks based on orthogonal cell stimuli.