Cationic Conjugated Polymers-Induced Quorum Sensing of Bacteria Cells
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- 作者：Pengbo Zhang ; Huan Lu ; Hui Chen ; Jiangyan Zhang ; Libing Liu ; Fengting Lv ; Shu Wang
- 刊名：Analytical Chemistry
- 出版年：2016
- 出版时间：March 15, 2016
- 年：2016
- 卷：88
- 期：6
- 页码：2985-2988
- 全文大小：319K
- ISSN：1520-6882
文摘
Bacteria quorum sensing (QS) has attracted significant interest for understanding cell–cell communication and regulating biological functions. In this work, we demonstrate that water-soluble cationic conjugated polymers (PFP-G2) can interact with bacteria to form aggregates through electrostatic interactions. With bacteria coated in the aggregate, PFP-G2 can induce the bacteria QS system and prolong the time duration of QS signal molecules (autoinducer-2 (AI-2)) production. The prolonged AI-2 can bind with specific protein and continuously regulate downstream gene expression. Consequently, the bacteria show a higher survival rate against antibiotics, resulting in decreased antimicrobial susceptibility. Also, AI-2 induced by PFP-G2 can stimulate 55.54 ± 12.03% more biofilm in E. coli. This method can be used to understand cell–cell communication and regulate biological functions, such as the production of signaling molecules, antibiotics, other microbial metabolites, and even virulence.