Facile Synthesis of Biocompatible Fluorescent Nanoparticles for Cellular Imaging and Targeted Detection of Cancer Cells

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• 作者：Fu Tang ; Chun Wang ; Xiaoyu Wang ; Lidong Li
• 刊名：ACS Applied Materials & Interfaces
• 出版年：2015
• 出版时间：November 18, 2015
• 年：2015
• 卷：7
• 期：45
• 页码：25077-25083
• 全文大小：368K
• ISSN：1944-8252

文摘

In this work, we report the facile synthesis of functional core鈥搒hell structured nanoparticles with fluorescence enhancement, which show specific targeting of cancer cells. Biopolymer poly-l-lysine was used to coat the silver core with various shell thicknesses. Then, the nanoparticles were functionalized with folic acid as a targeting agent for folic acid receptor. The metal-enhanced fluorescence effect was observed when the fluorophore (5-(and-6)-carboxyfluorescein-succinimidyl ester) was conjugated to the modified nanoparticle surface. Cellular imaging assay of the nanoparticles in folic acid receptor-positive cancer cells showed their excellent biocompatibility and selectivity. The as-prepared functional nanoparticles demonstrate the efficiency of the metal-enhanced fluorescence effect and provide an alternative approach for the cellular imaging and targeting of cancer cells.