Nanoparticle鈥揇rug Bioconjugate as Dual Functional Affinity Ligand for Rapid Point-of-Care Detection of Endotoxin in Water and Serum

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• 作者：Prasanta Kalita ; Anshuman Dasgupta ; Venkataraman Sritharan ; Shalini Gupta
• 刊名：Analytical Chemistry
• 出版年：2015
• 出版时间：November 3, 2015
• 年：2015
• 卷：87
• 期：21
• 页码：11007-11012
• 全文大小：321K
• ISSN：1520-6882

文摘

Endotoxin or lipopolysaccharide (LPS) is a major constituent of the Gram-negative bacterial cell wall that causes a life-threatening disorder called septicemia resulting from the unregulated activation of the innate immune system. We demonstrate a simple and robust drug-assisted dot blot bioassay for endotoxin detection that can be used right by the critically ill patients鈥?bedside. Target LPS molecules are trapped from serum or water on glass substrates via long-chain alkyls and tagged with reporter gold nanoparticles (NPs) preconjugated to an antibiotic drug called polymyxin B sulfate (PMB). A post-silver-enhancement step enables signal visibility to the bare eye over a wide and clinically relevant concentration range of 50 fg/mL鈥?0 ng/mL, allowing effortless diagnosis of sepsis at various stages, from early sepsis to septic shock.