Efficient Interface for Online Coupling of Capillary Electrophoresis with Inductively Coupled Plasma鈥揗ass Spectrometry and Its Application in Simultaneous Speciation Analysis of Arsenic and Selenium
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- 作者：Lihong Liu ; Zhaojun Yun ; Bin He ; Guibin Jiang
- 刊名：Analytical Chemistry
- 出版年：2014
- 出版时间：August 19, 2014
- 年：2014
- 卷：86
- 期：16
- 页码：8167-8175
- 全文大小：434K
- ISSN：1520-6882
文摘
A simple and highly efficient online system coupling of capillary electrophoresis to inductively coupled plasma鈥搈ass spectrometry (CE-ICP-MS) for simultaneous separation and determination of arsenic and selenium compounds was developed. CE was coupled to an ICP-MS system by a sprayer with a novel direct-injection high-efficiency nebulizer (DIHEN) chamber as the interface. By using this interface, six arsenic species, including arsenite (As(III), arsenate (As(V)), monomethylarsonic acid (MMA), dimethylarsinic acid (DMA), arsenobetaine (AsB), and arsenocholine (AsC) and five selenium species (such as sodium selenite (Se(IV)), sodium selenate (Se(VI)), selenocysteine (SeCys), selenomethionine (SeMet), and Se-methylselenocysteine (MeSeCys)) were baseline-separated and determined in a single run within 9 min under the optimized conditions. Minimum dead volume, low and steady sheath flow liquid, high nebulization efficiency, and high sample transport efficiency were obtained by using this interface. Detection limits were in the range of 0.11鈥?.37 渭g L鈥? for the six arsenic compounds (determined as 75As at m/z 75) and 1.33鈥?.31 渭g L鈥? for the five selenium species (determined as 82Se at m/z 82). Repeatability expressed as the relative standard deviations (RSD, n = 6) of both migration time and peak area were better than 2.68% for arsenic compounds and 3.28% for selenium compounds, respectively. The proposed method had been successfully applied for the determination of arsenic and selenium species in the certified reference materials DORM-3, water, urine, and fish samples.