Improved Analysis for Determining Diffusion Coefficients from Short, Single-Molecule Trajectories with Photoblinking

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• 作者：Bo Shuang ; Chad P. Byers ; Lydia Kisley ; Lin-Yung Wang ; Julia Zhao ; Hiroyuki Morimura ; Stephan Link ; Christy F. Landes
• 刊名：Langmuir
• 出版年：2013
• 出版时间：January 8, 2013
• 年：2013
• 卷：29
• 期：1
• 页码：228-234
• 全文大小：398K
• 年卷期：v.29,no.1(January 8, 2013)
• ISSN：1520-5827

文摘

Two maximum likelihood estimation (MLE) methods were developed for optimizing the analysis of single-molecule trajectories that include phenomena such as experimental noise, photoblinking, photobleaching, and translation or rotation out of the collection plane. In particular, short, single-molecule trajectories with photoblinking were studied, and our method was compared to existing analytical techniques applied to simulated data. The optimal method for various experimental cases was established, and the optimized MLE method was applied to a real experimental system: single-molecule diffusion of fluorescent molecular machines known as nanocars.