Large-Scale Production of Microcrystals and Precipitates of Proteins and Their Complexes

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• 作者：Monique Chan-Huot ; Luminita Duma ; Jean-Baptiste Charbonnier ; Jose-Enrique Herbert-Pucheta ; Liliane Assairi ; Yves Blouquit ; Daniel Abergel ; Geoffrey Bodenhausen
• 刊名：Crystal Growth & Design
• 出版年：2012
• 出版时间：December 5, 2012
• 年：2012
• 卷：12
• 期：12
• 页码：6199-6207
• 全文大小：457K
• 年卷期：v.12,no.12(December 5, 2012)
• ISSN：1528-7505

文摘

The optimum conditions for the formation of plate-like and urchin-like microcrystals of biomolecules and their transfer to rotors for solid-state NMR spectroscopy depend on a variety of factors, of which minimizing the manipulation of the microcrystals and storing the sample for several months at 277 K (4 掳C) play an important role. Three biological systems were investigated: Hen Egg-White (HEW) lysozyme (129 residues), the lengthened C-terminal domain (LCter) of Human centrin 2 (89 residues), and the complex between the C-terminal domain (Cter) of Human centrin 2 (79 residues) and the P17-XPC peptide (17 residues).