Fisetin Inhibits Lipopolysaccharide-Induced Macrophage Activation and Dendritic Cell Maturation
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- 作者：Sheng-Hung Liu ; Chao-Hsiung Lin ; Shih-Kai Hung ; Jen-Hwey Chou ; Chin-Wen Chi ; Shu-Ling Fu
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2010
- 出版时间：October 27, 2010
- 年：2010
- 卷：58
- 期：20
- 页码：10831-10839
- 全文大小：968K
- 年卷期：v.58,no.20(October 27, 2010)
- ISSN：1520-5118
文摘
Macrophages and dendritic cells are required for initiating innate immunity and adaptive immunity. Aberrant activation of macrophages and dendritic cells can cause detrimental immune responses; thus, agents effectively modulating their functions are of great clinical value. We herein investigated whether fisetin, a flavonoid prevalently present in fruits and vegetables, could inhibit macrophage activation and dendritic cell maturation. Fisetin suppressed LPS-induced NF-κB activation, expression of pro-inflammatory proteins (TNF-α and iNOS), MMP-9 activity, and phagocytic activity in macrophages. Furthermore, upon LPS-induced dendritic cell maturation, fisetin at nontoxic concentrations suppressed the expression of costimulatory molecules (CD80 and CD86), the production of cytokines (IL-12, IL-6, and TNF-α), and the endocytic activity of dendritic cells. Fisetin treatment significantly attenuated migration of dendritic cells into spleens and dendritic cell-mediated T cell activation in LPS-treated mice. Collectively, our data reveal that fisetin inhibits macrophage activation and impairs functional maturation of dendritic cells.