Food Byproducts as a New and Cheap Source of Bioactive Compounds: Lignans with Antioxidant and Anti-inflammatory Properties from Crataegus pinnatifida Seeds

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• 作者：Xiao-Xiao Huang ; Ming Bai ; Le Zhou ; Li-Li Lou ; Qing-Bo Liu ; Yan Zhang ; Ling-Zhi Li ; Shao-Jiang Song
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2015
• 出版时间：August 19, 2015
• 年：2015
• 卷：63
• 期：32
• 页码：7252-7260
• 全文大小：308K
• ISSN：1520-5118

文摘

During the process of manufacturing hawthorn (Crataegus pinnatifida) juice and jam, a significant quantity of byproducts (leaves, seeds) is generated. The antioxidant and anti-inflammatory bioassay-guided fractionation of the extract of hawthorn seeds has led to the isolation of eight new lignans, hawthornnins A鈥揌 (1鈥?b>8), and seven known analogues (9鈥?b>15). Their structures were elucidated by spectroscopic techniques, including 1D and 2D NMR and CD spectra. The radical-scavenging effects of all isolated compounds were investigated. 1鈥?b>6 and 8 showed moderate activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH), whereas 1鈥?b>6 and 14 displayed good 2,2鈥?azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) free radical-scavenging activities that were even more potent than that of trolox. In addition, all isolates were evaluated for their anti-inflammatory activities by detecting the nitric oxide (NO) and tumor necrosis factor 伪 (TNF-伪) production by the LPS-induced murine macrophage cell line RAW264.7, and compounds 1鈥?b>7, 13, and 14 exhibited potent inhibition of NO and TNF-伪 production. The structure鈥揳ctivity relationships of isolated lignans were also examined, and the results obtained show that C. pinnatifida seeds can be regarded as a potential new and cheap source of antioxidants and inflammation inhibitors.