文摘
In this paper, we report on the Ag nanoparticle-containing hybrid poly(N-isopropylacrylamide-co-acrylic acid) (PNIPAM-co-PAA) microgels with pH- and thermoresponsive metal-enhanced fluorescence (MEF). The hybrid microgels were prepared by in situ reducing Ag salts to Ag nanoparticles in the PNIPAM-co-PAA microgels. According to the interaction distance-dependent nature of MEF effects, we can realize a controllable MEF effect by adjusting the average interaction distance between fluorophores and Ag nanoparticles due to the good stimuli-responsive swelling鈥搒hrinking behavior of the hybrid microgels. The results show that MEF effect can be well tuned in the pH region 2鈥?2 as well as the temperature region of 20鈥?0 掳C. By this method, an enhanced fluorescence detection can possibly be manipulated by adjusting external stimuli such as pH and temperature.