Hemoglobin-Based Oxygen Carrier Microparticles: Synthesis, Properties, and In Vitro and In Vivo Investigations

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• 作者：Yu Xiong ; Axel Steffen ; Kristin Andreas ; Susanne M眉ller ; Nadine Sternberg ; Radostina Georgieva ; Hans B盲umler
• 刊名：Biomacromolecules
• 出版年：2012
• 出版时间：October 8, 2012
• 年：2012
• 卷：13
• 期：10
• 页码：3292-3300
• 全文大小：472K
• 年卷期：v.13,no.10(October 8, 2012)
• ISSN：1526-4602

文摘

Bovine hemoglobin microparticles (Hb-MPs) as suitable oxygen carriers are fabricated easily by three key steps: coprecipitation of Hb and CaCO₃ to make Hb-CaCO₃-microparticles (Hb-CaCO₃-MPs), cross-linking by glutaraldehyde (GA) to polymerize the Hb and dissolution of CaCO₃ template to obtain pure Hb-MPs. The Hb entrapment efficiency ranged from 8 to 50% corresponding to a hemoglobin quantity per Hb-MP of at least one-third of that in one erythrocyte. The Hb-MPs are spherical, with an average diameter of 3.2 渭m and high oxygen affinity. The methemoglobin level was increased after preparation, but can be reduced to less than 7% with ascorbic acid. Phagocytosis assays showed low immunogenicity of Hb-MPs if the particles were cross-linked with low concentration of GA and treated with sodium borohydride. Magnetite-loaded Hb-MPs circulated up to 4 days after intravenous application.