Characterization of the Glycosyltransferase Activity of DesVII: Analysis of and Implications for the Biosynthesis of Macrolide Antibiotics
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- 作者：Svetlana A. Borisova ; Lishan Zhao ; Charles E. Melanç ; on III ; Chai-Lin Kao ; and Hung-wen Liu
- 刊名：Journal of the American Chemical Society
- 出版年：2004
- 出版时间：June 2, 2004
- 年：2004
- 卷：126
- 期：21
- 页码：6534 - 6535
- 全文大小：42K
- 年卷期：v.126,no.21(June 2, 2004)
- ISSN：1520-5126
文摘
In vitro catalytic activity of DesVII, the glycosyltransferase involved in the biosynthesis of methymycin, neomethymycin, narbomycin, and pikromycin in Streptomyces venezuelae, is described. This is the first report of demonstrated in vitro activity of a glycosyltransferase involved in the biosynthesis of macrolide antibiotics. DesVII is unique among glycosyltransferases in that it requires an additional protein component, DesVIII, as well as basic pH for its full activity.