This paper summarizes the recent acti
vities of the Chromosome-Centric Human Proteome Project (C-HPP) consortium, which de
velops new technologies to identify yet-to-be annotated proteins (termed 鈥渕issing proteins鈥? in biological samples that lack sufficient experimental e
vidence at the protein le
vel for confident protein identification. The C-HPP also aims to identify new protein forms that may be caused by genetic
variability, post-translational modifications, and alternati
ve splicing. Proteogenomic data integration forms the basis of the C-HPP鈥檚 acti
vities; therefore, we ha
ve summarized some of the key approaches and their roles in the project. We present new analytical technologies that impro
ve the chemical space and lower detection limits coupled to bioinformatics tools and some publicly a
vailable resources that can be used to impro
ve data analysis or support the de
velopment of analytical assays. Most of this paper鈥檚 content has been compiled from posters, slides, and discussions presented in the series of C-HPP workshops held during 2014. All data (posters, presentations) used are a
vailable at the C-HPP Wiki (
http://c-hpp.webhosting.rug.nl/) and in the Supporting Information.