Transport of Antihypertensive Peptide RVPSL, Ovotransferrin 328鈥?32, in Human Intestinal Caco-2 Cell Monolayers
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- 作者：Long Ding ; Liying Wang ; Yan Zhang ; Jingbo Liu
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2015
- 出版时间：September 23, 2015
- 年：2015
- 卷：63
- 期：37
- 页码：8143-8150
- 全文大小：300K
- ISSN：1520-5118
文摘
The objective of this study was to investigate the transepithelial transport of RVPSL (Arg-Val-Pro-Ser-Leu), an egg-white-derived peptide with angiotensin I-converting enzyme (ACE) inhibitory and antihypertensive activity, in human intestinal Caco-2 cell monolayers. Results revealed that RVPSL could be passively transported across Caco-2 cell monolayers. However, during the process of transport, 36.31% 卤 1.22% of the initial RVPSL added to the apical side was degraded, but this degradation decreased to 23.49% 卤 0.68% when the Caco-2 cell monolayers were preincubated with diprotin A (P < 0.001), suggesting that RVPSL had a low resistance to various brush border membrane peptidases. When transport from the apical side to the basolateral side was investigated, the apparent permeability coefficient (Papp) was (6.97 卤 1.11) 脳 10鈥? cm/s. The transport route of RVPSL appears to be the paracellular pathway via tight junctions, as only cytochalasin D, a disruptor of tight junctions (TJs), significantly increased the transport rate (P < 0.001). In addition, the relationship between the structure of RVPSL and transport across Caco-2 cell monolayers was studied by mutation of RVPSL. It was found that N-terminal Pro residues were more beneficial for transport of pentapeptides across Caco-2 cell monolayers than Arg and Val. Furthermore, RVPSL could be more easily transported as smaller peptides, especially in the form of dipeptides and tripeptides.