Identification of New Fyn Kinase Inhibitors Using a FLAP-Based Approach

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• 作者：Giulio Poli ; Tiziano Tuccinardi ; Flavio Rizzolio ; Isabella Caligiuri ; Lorenzo Botta ; Carlotta Granchi ; Gabriella Ortore ; Filippo Minutolo ; Silvia Schenone ; Adriano Martinelli
• 刊名：Journal of Chemical Information and Modeling
• 出版年：2013
• 出版时间：October 28, 2013
• 年：2013
• 卷：53
• 期：10
• 页码：2538-2547
• 全文大小：477K
• 年卷期：v.53,no.10(October 28, 2013)
• ISSN：1549-960X

文摘

The abnormal activity of Fyn tyrosine kinase has been shown to be related to various human cancers. Furthermore, its involvement in signaling pathways that lead to severe pathologies, such as Alzheimer鈥檚 and Parkinson鈥檚 diseases, has also been demonstrated, thus making Fyn an attractive target for the discovery of potential novel therapeutics for brain pathologies and tumors. In this study we evaluated the reliability of various screening approaches based on the FLAP software. By the application of the best procedure, the virtual screening workflow was used to filter the Gold and Platinum database from Asinex to identify new Fyn inhibitors. Enzymatic assays revealed that among the eight top-scoring compounds five proved to efficiently inhibit Fyn activity with IC₅₀ values in the micromolar range. These results demonstrate the validity of the methodologies we followed. Furthermore, the five active compounds herein described may be considered as interesting leads for the development of new and more efficient Fyn inhibitors.