Directed Assembly of DNA-Functionalized Gold Nanoparticles Using Pyrrole鈥揑midazole Polyamides

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• 作者：沤eljka Krpeti膰 ; Ishwar Singh ; Wu Su ; Luca Guerrini ; Karen Faulds ; Glenn A. Burley ; Duncan Graham
• 刊名：The Journal of the American Chemical Society
• 出版年：2012
• 出版时间：May 23, 2012
• 年：2012
• 卷：134
• 期：20
• 页码：8356-8359
• 全文大小：290K
• 年卷期：v.134,no.20(May 23, 2012)
• ISSN：1520-5126

文摘

Traditional methods for the construction of nanoparticle arrays and lattices exploit Watson鈥揅rick base pairing of single-stranded DNA sequences as a proxy for self-assembly. Although this approach has been utilized in a variety of applications in nanoassembly, diagnostics, and biomedicine, the diversity of this recognition lexicon could be considerably increased by developing strategies that recognize the base-pairing landscape of double-stranded DNA (dsDNA) sequences. Herein we describe the first report of programmed gold nanoparticle (GNP) aggregation directed by the recognition of dsDNA sequences using pyrrole鈥搃midazole polyamide鈥揋NP (PA鈥揋NP) conjugates. We demonstrate the reversibility and selectivity of this strategy for forming GNP aggregates in the presence of fully matched dsDNA sequences relative to dsDNA sequences containing one- and two-base-pair mismatches.