Anti-inflammatory Effect of Methyl Gallate on Experimental Arthritis: Inhibition of Neutrophil Recruitment, Production of Inflammatory Mediators, and Activation of Macrophages

详细信息    查看全文

• 作者：Luana Barbosa Correa ; Tatiana Almeida Pádua ; Leonardo Noboru Seito ; Thadeu Estevam Moreira Maramaldo Costa ; Magaiver Andrade Silva ; André Luis Peixoto Candéa ; Elaine Cruz Rosas ; Maria G. Henriques
• 刊名：Journal of Natural Products
• 出版年：2016
• 出版时间：June 24, 2016
• 年：2016
• 卷：79
• 期：6
• 页码：1554-1566
• 全文大小：788K
• 年卷期：0
• ISSN：1520-6025

文摘

Methyl gallate (MG) is a prevalent phenolic acid in the plant kingdom, and its presence in herbal medicines might be related to its remarkable biological effects, such as its antioxidant, antitumor, and antimicrobial activities. Although some indirect evidence suggests anti-inflammatory activity for MG, there are no studies demonstrating this effect in animal models. Herein, we demonstrated that MG (0.7–70 mg/kg) inhibited zymosan-induced experimental arthritis in a dose-dependent manner. The oral administration of MG (7 mg/kg) attenuates arthritis induced by zymosan, affecting edema formation, leukocyte migration, and the production of inflammatory mediators (IL-1β, IL-6, TNF-α, CXCL-1, LTB₄, and PGE₂). Pretreatment with MG inhibited in vitro neutrophil chemotaxis elicited by CXCL-1, as well as the adhesion of these cells to TNF-α-primed endothelial cells. MG also impaired zymosan-stimulated macrophages by inhibiting IL-6 and NO production, COX-2 and iNOS expression, and intracellular calcium mobilization. Thus, MG is likely to present an anti-inflammatory effect by targeting multiple cellular events such as the production of various inflammatory mediators, as well as leukocyte activation and migration.