Study of Zalema Grape Pomace: Phenolic Composition and Biological Effects in Caenorhabditis elegans
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- 作者：M. Jos茅 Jara-Palacios ; Susana Gonz谩lez-Manzano ; M. Luisa Escudero-Gilete ; Dolores Hernanz ; Montserrat Due帽as ; Ana M. Gonz谩lez-Param谩s ; Francisco J. Heredia ; Celestino Santos-Buelga
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2013
- 出版时间：May 29, 2013
- 年：2013
- 卷：61
- 期：21
- 页码：5114-5121
- 全文大小：285K
- 年卷期：v.61,no.21(May 29, 2013)
- ISSN：1520-5118
文摘
The phenolic composition of the extractable fraction of Zalema grape pomace has been analyzed by HPLC-DAD-MS and consisted of mainly flavanols and flavonols (122.75 and 23.11 mg/100 g dry pomace, respectively). The antioxidant activity has been determined by in vitro FRAP, ABTS, and ORAC assays (11.7, 34.9, and 63.6 mmol of Trolox equivalents (TE) per 100 g of dry pomace, respectively) and in vivo using the model organism Caenorhabditis elegans. Cultivation of C. elegans in media containing 100 渭g/mL dry pomace extract increased the survival of worms submitted to thermally induced oxidative stress, whereas a decrease in the rate of worm survival was found for 300 渭g/mL extract. Interestingly, the levels of reactive oxygen species (ROS) were significantly decreased in stressed worms treated with the pomace extract at the two concentration levels. Further studies are required to explain this unexpected behavior, as well as to determine the compounds and mechanisms involved in the observed effects.