Feasibility of Polysaccharide Hybrid Materials for Scaffolds in Cartilage Tissue Engineering: Evaluation of Chondrocyte Adhesion to Polyion Complex Fibers Prepared from Alginate and Chitosan

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• 作者：Norimasa Iwasaki ; Shin-Tarou Yamane ; Tokifumi Majima ; Yasuhiko Kasahara ; Akio Minami ; Kazuo Harada ; Sachiko Nonaka ; Nobuhiko Maekawa ; Hiroshi Tamura ; Seiichi Tokura ; Masamichi Shiono ; Kenji Monde ; and
• 刊名：Biomacromolecules
• 出版年：2004
• 出版时间：May 2004
• 年：2004
• 卷：5
• 期：3
• 页码：828 - 833
• 全文大小：157K
• 年卷期：v.5,no.3(May 2004)
• ISSN：1526-4602

文摘

The ideal cell-carrier material for cartilage regeneration should be one that closely mimics the naturalenvironment in a living articular cartilage matrix. In the current study, we considered that alginate-basedchitosan hybrid biomaterials could provide excellent supports for chondrocyte adhesion. To test this hypothesis,we investigated the adhesion behavior of rabbit chondrocytes onto an alginate polymer versus the adhesionof the chondrocytes onto some alginate-based chitosan hybrid polymer fibers in vitro. We demonstratedthat the alginate-based chitosan hybrid polymer fibers showed much improved adhesion capacity withchondrocytes in comparison with alginate polymer fiber. Additionally, morphologic studies revealedmaintenance of the characteristic round morphology of the chondrocyte and the dense fiber of the type IIcollagen produced by the chondrocytes in the hybrid polymer. On the basis of these results, we concludethat an alginate-based chitosan hybrid polymer fiber has considerable potential as a desirable biomaterialfor cartilage tissue scaffolds.