Luminescent Silica Nanobeads: Characterization and Evaluation as Efficient Cytoplasmatic Transporters for T-Lymphocytes
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- 作者：Massimo Bottini ; Fabio Cerignoli ; David M. Mills ; Federica D'Annibale ; Marilisa Leone ; Nicola Rosato ; Andrea Magrini ; Maurizio Pellecchia ; Antonio Bergamaschi ; Tomas Mustelin
- 刊名：Journal of the American Chemical Society
- 出版年：2007
- 出版时间：June 27, 2007
- 年：2007
- 卷：129
- 期：25
- 页码：7814 - 7823
- 全文大小：510K
- 年卷期：v.129,no.25(June 27, 2007)
- ISSN：1520-5126
文摘
We report the fabrication and characterization of neutravidin-conjugated silica nanobeads dopedwith a ruthenium-complex luminophore and functionalized with antihuman CD3, antihuman CD28, and anacid-sensitive polymer. We observed that the nanobeads were readily delivered into Jurkat T leukemiacells by endocytosis, transported into lysosomes and subsequently into the cytoplasm as revealed by pH-sensitive luminescence. Since signs of cytotoxicity were not observed, the reported nanobeads could bean excellent and nontoxic building block for efficient intracellular transporters.