Absorption, Disposition, Metabolism, and Excretion of [3-14C]Caffeic Acid in Rats

详细信息    查看全文

• 作者：Maizatul H. Omar ; William Mullen ; Angelique Stalmach ; Cyril Auger ; Jean-Max Rouanet ; Pierre-Louis Teissedre ; Stuart T. Caldwell ; Richard C. Hartley ; Alan Crozier
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2012
• 出版时间：May 23, 2012
• 年：2012
• 卷：60
• 期：20
• 页码：5205-5214
• 全文大小：465K
• 年卷期：v.60,no.20(May 23, 2012)
• ISSN：1520-5118

文摘

Male Sprague鈥揇awley rats ingested 140 脳 10⁶ dpm of [3-¹⁴C]trans-caffeic acid, and over the ensuing 72 h period, body tissues, plasma, urine, and feces were collected and the overall levels of radioactivity determined. Where sufficient radioactivity had accumulated, samples were analyzed by HPLC with online radioactivity and tandem mass spectrometric detection. Nine labeled compounds were identified, the substrate and its cis isomer, 3鈥?O- and 4鈥?O-sulfates and glucuronides of caffeic acid, 4鈥?O-sulfates and glucuronides of ferulic acid, and isoferulic acid-4鈥?O-sulfate. Four unidentified metabolites were also detected. After passing down the gastrointestinal tract, the majority of the radiolabeled metabolites were excreted in urine with minimal accumulation in plasma. Only relatively small amounts of an unidentified ¹⁴C-labeled metabolite were expelled in feces. There was little or no accumulation of radioactivity in body tissues, including the brain. The overall recovery of radioactivity 72 h after ingestion of [3-¹⁴C]caffeic acid was 80% of intake.
<h4>Keywords: h4> [3-¹⁴C]caffeic acid; absorption; disposition; metabolism; excretion; rats