Characterization of a Novel Polysaccharide from Tetraploid Gynostemma pentaphyllum Makino
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- 作者：Yuge Niu ; Wei Yan ; Junli Lv ; Wenbing Yao ; Liangli (Lucy) Yu
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2013
- 出版时间：May 22, 2013
- 年：2013
- 卷：61
- 期：20
- 页码：4882-4889
- 全文大小：318K
- 年卷期：v.61,no.20(May 22, 2013)
- ISSN：1520-5118
文摘
A novel heteropolysaccharide (GPP-TL) was isolated from tetraploid Gynostemma pentaphyllum (Makino) leaf by hot water extraction and anion-exchange and gel permeation chromatography approaches. GPP-TL had a molecular weight of 9.3 脳 103 Da and was primarily composed of glucose, galactose, and arabinose, with a molar ratio of 43:5:1, respectively. The chemical structure of GPP-TL was characterized using chemical and instrumental analyses. The results indicated the presence of (1鈫?)-伪-d-glucopyranosyl, (1鈫?)-尾-d-galactopyranosyl, (1鈫?,6)-linked-伪-d-glucopyranosyl, and terminal 1鈫?-伪-d-glucopyranosyl moieties in a molar ratio of 5.7:1:1.5:1, respectively. The results indicated that GPP-TL had glucose and galactose residues in the main chain with (1鈫?)-linked branches at glucose residues. In addition, GPP-TL exhibited scavenging capacities against hydroxyl, peroxyl, and DPPH radicals in vitro and had a stronger bile acid-binding ability than psyllium on a same-weight basis.