Application of Thiolated Gold Nanoparticles for the Enhancement of Glucose Oxidase Activity

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• 作者：Pratibha Pandey ; Surinder P. Singh ; Sunil K. Arya ; Vinay Gupta ; Monika Datta ; Sukhvir Singh ; Bansi D. Malhotra
• 刊名：Langmuir
• 出版年：2007
• 出版时间：March 13, 2007
• 年：2007
• 卷：23
• 期：6
• 页码：3333 - 3337
• 全文大小：263K
• 年卷期：v.23,no.6(March 13, 2007)
• ISSN：1520-5827

文摘

Glucose oxidase (GO_x) has been covalently immobilized onto chemically synthesized thiolated gold nanoparticles(5-8 nm) via N-ethyl-N'-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS). The lowervalue of the Michaelis-Menton constant obtained for the immobilized (3.74 mM) GO_x compared with that for thefree (5.85 mM) GO_x suggests significant enhancement in the activity of GO_x attached to thiolated gold nanoparticles.The covalently immobilized GO_x thiolated nanoparticles exhibit a response time of 30 s, a shelf life of more than 6months, and improved tolerance to both pH and temperature.