Quorum-Sensing-Based Toolbox for Regulatable Transgene and siRNA Expression in Mammalian Cells

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• 作者：Wilfried Weber ; Laetitia Malphettes ; Matthias Rinderknecht ; Ronald G. Schoenmakers ; Manuela Spielmann ; Bettina Keller ; Petra van de Wetering ; Cornelia C. Weber ; and Martin Fussenegger
• 刊名：Biotechnology Progress
• 出版年：2005
• 出版时间：February 2005
• 年：2005
• 卷：21
• 期：1
• 页码：178 - 185
• 全文大小：162K
• 年卷期：v.21,no.1(February 2005)
• ISSN：1520-6033

文摘

Technologies for regulated expression of multiple transgenes in mammalian cells havegathered momentum for bioengineering, gene therapy, drug discovery, and gene-function analyses. Capitalizing on recently developed mammalian transgene modalities(QuoRex) derived from Streptomyces coelicolor, we have designed a flexible and highlycompatible expression vector set that enables desired transgene/siRNA control inresponse to the nontoxic butyrolactone SCB1. The construction-kit-like expressionportfolio includes (i) multicistronic (pTRIDENT), (ii) autoregulated, (iii) bidirectional(pBiRex), (iv) oncoretro- and lentiviral transduction, and (v) RNA polymerase II-basedsiRNA transcription-fine-tuning vectors for straightforward implementation of QuoRex-controlled (trans)gene modulation in mammalian cells.