Creation of Cross-Linked Bilayer Membranes That Can Incorporate Membrane Proteins from Oligo-Asp-Based Peptide Gemini Surfactants

详细信息    查看全文

• 作者：Shuhei Koeda ; Katsunari Umezaki ; Ayumi Sumino ; Tomoyasu Noji ; Atsushi Ikeda ; Yasushi Yamamoto ; Takehisa Dewa ; Keijiro Taga ; Mamoru Nango ; Toshiki Tanaka ; Toshihisa Mizuno
• 刊名：Langmuir
• 出版年：2013
• 出版时间：September 17, 2013
• 年：2013
• 卷：29
• 期：37
• 页码：11695-11704
• 全文大小：602K
• 年卷期：v.29,no.37(September 17, 2013)
• ISSN：1520-5827

文摘

We designed novel bilayer-forming amphiphiles based on the cyclic oligo-Asp-based peptide gemini (PG) surfactants cr-D₂C₁₂ and cr-D₃C₁₂, which consist of 鈭扖ys(Asp)_nCys鈥?(n = 2 or 3) as a core peptide and two Cys residues containing a dodecylamidomethyl group. Dynamic light scattering and transmission electron microscopy measurements revealed the formation of spherical bilayer membranes that could incorporate the light-harvesting antenna complex 2 (LH2) from Rhodopseudomonas acidophila. Furthermore, this proteoliposome-like conjugate could be assembled onto cationized glass and mica to form planar bilayer membranes incorporating LH2. Using atomic force microscopy, we observed LH2 protruding (ca. 1.2鈥?.5 nm) from flat terraces of the planar bilayer membranes formed from cr-D₂C₁₂ or cr-D₃C₁₂. Thus, our designed PG surfactants are a new class of bilayer-forming amphiphiles that may be applied to the study of various membrane proteins.