Surface-Derivatized Nanoceria with Human Carbonic Anhydrase II Inhibitors and Fluorophores: A Potential Drug Delivery Device
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- 作者：Swanand Patil ; Serge Reshetnikov ; Manas K. Haldar ; Sudipta Seal ; Sanku Mallik
- 刊名：Journal of Physical Chemistry C
- 出版年：2007
- 出版时间：June 21, 2007
- 年：2007
- 卷：111
- 期：24
- 页码：8437 - 8442
- 全文大小：504K
- 年卷期：v.111,no.24(June 21, 2007)
- ISSN：1932-7455
文摘
Human carbonic anhydrase (hCAII) is a metalloenzyme that catalyzes the reversible hydration of carbondioxide to bicarbonate and is associated with glaucoma (a major cause of blindness). The present study focuseson the use of cerium oxide nanoparticles (nanoceria) as a potential delivery device for hCAII inhibitors.Carboxybenzenesulfonamide, an inhibitor of the hCAII enzyme, was attached to nanoceria particles usingepichlorohydrin as an intermediate linkage. Along with the CA inhibitor, a fluorophore (carboxyfluorescein)was also attached on the nanoparticles to enable the tracking of the nanoparticles in vitro as well as in vivo.X-ray photoelectron spectroscopic studies carried out at each reaction step confirmed the successfulderivatization of the nanoceria particles. The attachment of carboxyfluorescein was also confirmed by confocalfluorescence microscopy. Preliminary studies suggest that carboxybenzenesulfonamide-functionalized nanoceriaretains its inhibitory potency for hCAII.