Encapsidated Atom-Transfer Radical Polymerization in Q尾 Virus-like Nanoparticles
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- 作者：Marisa L. Hovlid ; Jolene L. Lau ; Kurt Breitenkamp ; Cody J. Higginson ; Burkhardt Laufer ; Marianne Manchester ; M. G. Finn
- 刊名：ACS Nano
- 出版年：2014
- 出版时间：August 26, 2014
- 年：2014
- 卷：8
- 期：8
- 页码：8003-8014
- 全文大小：860K
- ISSN：1936-086X
文摘
Virus-like particles (VLPs) are unique macromolecular structures that hold great promise in biomedical and biomaterial applications. The interior of the 30 nm-diameter Q尾 VLP was functionalized by a three-step process: (1) hydrolytic removal of endogenously packaged RNA, (2) covalent attachment of initiator molecules to unnatural amino acid residues located on the interior capsid surface, and (3) atom-transfer radical polymerization of tertiary amine-bearing methacrylate monomers. The resulting polymer-containing particles were moderately expanded in size; however, biotin-derivatized polymer strands were only very weakly accessible to avidin, suggesting that most of the polymer was confined within the protein shell. The polymer-containing particles were also found to exhibit physical and chemical properties characteristic of positively charged nanostructures, including the ability to easily enter mammalian cells and deliver functional small interfering RNA.