Analytical Innovations in the Detection of Phenolics in Wines
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- 作者：Pietro Russo ; Á ; lvaro Andreu-Navarro ; Marí ; a-Paz Aguilar-Caballos ; Juan-Manuel Ferná ; ndez-Romero ; Agustina Gó ; mez-Hens
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2008
- 出版时间：March 26, 2008
- 年：2008
- 卷：56
- 期：6
- 页码：1858 - 1865
- 全文大小：362K
- 年卷期：v.56,no.6(March 26, 2008)
- ISSN：1520-5118
文摘
A liquid chromatographic method with online photometric and luminescent detection for the determination of 18 phenolic compounds in wines is reported. Photometric detection is performed at four wavelengths, namely, 256, 280, 320, and 365 nm, using a diode array detection system. The luminescent detection is achieved by means of a postcolumn derivatization reaction of 10 of these compounds with terbium(III) in the presence of synergistic agents, such as ethylenediaminetetraacetic acid (EDTA) and n-octyltriphosphine oxide (TOPO). A micellar medium provided by the surfactants sodium dodecyl sulfate and Triton X-100 was used for the determination of the luminescent chelates at λex 317 and λem 545 nm. The long wavelength emission of lanthanide chelates can minimize interferences from background sample matrix, which usually emit at shorter wavelengths. The analytical features of the photometric and fluorometric methods, such as dynamic ranges of the calibration graphs, detection limits, and precision data, have been obtained. The practical usefulness of the developed methods is demonstrated by the analysis of Spanish and Italian wine samples (red, rosé, oloroso, and white), which were diluted and directly injected into the chromatographic system. The accuracy of both methods was checked by assaying a recovery study, which was performed at three different analyte levels for each type of sample.