Automated Strong Cation Exchange Extraction of Fatty Acid Esters of 3-(N-Phenylamino)-1,2-propanediol from Oil Samples for Routine Quantification by HPLC-APCI/MS/MS

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• 作者：Rosa E. Calaf ; Juana Pe&ntilde ; a ; Sonia Paytubi ; Montserrat Carrascal ; Manuel Posada ; Emilio Gelpi ; and Joaquin Abian
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2001
• 出版时间：November 2001
• 年：2001
• 卷：49
• 期：11
• 页码：5085 - 5091
• 全文大小：115K
• 年卷期：v.49,no.11(November 2001)
• ISSN：1520-5118

文摘

Fatty acid esters of 3-(N-phenylamino)-1,2-propanediol are currently considered the best chemicalmarkers of toxic oils related to the Spanish toxic oil syndrome. Recent research in this area hasundertaken the exhaustive and quantitative characterization of these compounds in oils collectedduring the epidemic outbreak. Current methods developed in this laboratory are based on solidphase extraction (SPE) using SCX cartridges followed by HPLC-APCI/MS/MS quantification. Tocircumvent the long and tedious extraction procedure, the SPE protocol was adapted for automaticextraction and the problems derived from the use of the immiscible solvents required for the SCXextraction were solved. Linearity of the analytical method was found in the same range as for themanual method. Extraction recoveries were 87 and 75% for 2-hydroxy-3-(N-phenylamino)propyllinoleate and 2-(linoleyloxy)-3-(N-phenylamino)propyl linoleate, respectively, and the correspondingcoefficients of variation were ~1%, greatly improving reproducibility over manual procedures.Keywords: Toxic oil syndrome; fatty acid esters of 3-(N-phenylamino)-1,2-propanediol; strong cationexchange; HPLC-APCI/MS/MS