A procedure based on liquid chromato
graphy-massspectrometry (LC-MS) is described for determination ofamphetamine, methamphetamine, and methylendioxyderivatives in meconium, usin
g 3,4-methylendioxypropylamphetamine as internal standard. The analytes wereinitially extracted from the matrix by 17 mM methanolicHCl. Subsequently, a solid-phase extraction with BondelutCertify columns was applied. Chromato
graphy was performed on a C
18 reversed-phase column usin
g a linear
gradient of 10 mM ammonium bicarbonate, pH 9.0-methanol as a mobile phase. Analytes were determinedin LC-MS sin
gle ion monitorin
g mode with an atmospheric pressure ionization-electrospray interface. Themethod was validated in the ran
ge 0.005-1.00
![](/ima<font color=)
ges/entities/m
gr.
gif">
g/
gusin
g 1
g of meconium per assay. Mean recoveries ran
gedbetween 61.1 and 87.2% for different analytes. Thequantification limits were 0.005
![](/ima<font color=)
ges/entities/m
gr.
gif">
g/
g meconium foramphetamine, methamphetamine, and 4-hydroxy-3-methoxymethamphetamine and 0.004
![](/ima<font color=)
ges/entities/m
gr.
gif">
g/
g meconium for 3,4-methylenedioxyamphetamine, 3,4-methylenedioxymethamphetamine, 3,4-methylenedioxyethylamphetamine, and
N-methyl-1-(3,4-methylenedioxyphenyl)-2-butanamine. Themethod was applied to analysis of meconium in newbornsto assess eventual fetal exposure to amphetamine derivatives.