Candida rugosa Lipase-Catalyzed Intramolecular O- to N- Transacylation of Butyryl Propranolol in the Presence of Cyclodextrins

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• 作者：Rizette Á ; vila-Gonzá ; lez ; Manuela Pé ; rez-Gilabert ; Miguel A. L&oacute ; pez-L&oacute ; pez ; and Francisco Garcí ; a-Carmona
• 刊名：Biotechnology Progress
• 出版年：2005
• 出版时间：April 2005
• 年：2005
• 卷：21
• 期：2
• 页码：338 - 342
• 全文大小：89K
• 年卷期：v.21,no.2(April 2005)
• ISSN：1520-6033

文摘

In the present paper, a novel enzymatic reaction between (R,S)-O-butyryl propranolol(O-BP) and lipase from Candida rugosa in the presence of hydroxypropyl-ges/gifchars/beta2.gif" BORDER=0 ALIGN="middle">-cyclodextrin(HP-ges/gifchars/beta2.gif" BORDER=0 ALIGN="middle">-CD) is described. Under the used condition, lipase catalyzed the intramoleculartransacylation of O-BP into N-butyryl propranolol (N-BP). Propranolol, the productof the expected hydrolysis reaction, was not detected in the reaction medium. A chiralanalysis of the reaction product indicated that lipase showed a preference for (R)-O-butyryl propranolol since it first transformed the (R)-enantiomer and then thecorresponding (S)-enantiomer. The influence of different reaction conditions on theinitial rate is also studied.