Direct Imaging of DNA Fibers: The Visage of Double Helix
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- 作者：Francesco Gentile ; Manola Moretti ; Tania Limongi ; Andrea Falqui ; Giovanni Bertoni ; Alice Scarpellini ; Stefania Santoriello ; Luca Maragliano ; Remo Proietti Zaccaria ; Enzo di Fabrizio
- 刊名：Nano Letters
- 出版年：2012
- 出版时间：December 12, 2012
- 年：2012
- 卷：12
- 期：12
- 页码：6453-6458
- 全文大小：425K
- 年卷期：v.12,no.12(December 12, 2012)
- ISSN：1530-6992
文摘
Direct imaging becomes important when the knowledge at few/single molecule level is requested and where the diffraction does not allow to get structural and functional information. Here we report on the direct imaging of double stranded (ds) 位-DNA in the A conformation, obtained by combining a novel sample preparation method based on super hydrophobic DNA molecules self-aggregation process with transmission electron microscopy (TEM). The experimental breakthrough is the production of robust and highly ordered paired DNA nanofibers that allowed its direct TEM imaging and the double helix structure revealing.