Procedure for Radiolabeling Gizzerosine and Basis for a Radioimmunoassay
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- 作者：Marcela Torres ; Headdy Manosalva ; Isabel Carrasco ; Alfredo E. De Ioannes ; and Marí ; a Iné ; s Becker
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：1999
- 出版时间：October 1999
- 年：1999
- 卷：47
- 期：10
- 页码：4231 - 4236
- 全文大小：82K
- 年卷期：v.47,no.10(October 1999)
- ISSN：1520-5118
文摘
A method for the labeling of gizzerosine (GZ), a biogenic amine found in fish meal, is described. Thelabeling procedure with 125I using a water-soluble Bolton-Hunter reagent and a mild water-insolubleoxidant (Iodogen) reagent is rapid and reproducible. The 125I-GZ hapten was demonstrated to beimmunologically active in a radioimmunoassay developed with polyclonal antibodies to GZ absorbedwith a histamine-Sepharose column. The curves were linear in the range of 0.0001 and 0.1 
g/mL.Samples of fish meal previously extracted of histamine with methanol and submitted to acidhydrolysis were contaminated with known amounts of GZ and submitted to the assay. The fishmeal samples contaminated with GZ show a dose-response effect similar to the standard curve,and apparently the other component present in the sample did not interfere with the binding of theantibodies to 125I-GZ. These data indicate the suitability of the radioimmunoassay to determinespecifically GZ in fish meal.Keywords: Gizzerosine labeling; polyclonal antibodies; RIA
g/mL.Samples of fish meal previously extracted of histamine with methanol and submitted to acidhydrolysis were contaminated with known amounts of GZ and submitted to the assay. The fishmeal samples contaminated with GZ show a dose-response effect similar to the standard curve,and apparently the other component present in the sample did not interfere with the binding of theantibodies to 125I-GZ. These data indicate the suitability of the radioimmunoassay to determinespecifically GZ in fish meal.Keywords: Gizzerosine labeling; polyclonal antibodies; RIA