Osmotically Induced Morphological Changes of Extruded Dioctadecyldimethylammonium Chloride (DODAC) Dispersions

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• 作者：Pieter Saveyn ; Jan Cocquyt ; Paul Bomans ; Peter Frederik ; Marcel De Cuyper ; Paul Van der Meeren
• 刊名：Langmuir
• 出版年：2007
• 出版时间：April 24, 2007
• 年：2007
• 卷：23
• 期：9
• 页码：4775 - 4781
• 全文大小：427K
• 年卷期：v.23,no.9(April 24, 2007)
• ISSN：1520-5827

文摘

Extruded vesicles, which are often used as models for living cells, can change their morphology when they arediluted into a hyperosmotic medium. Different morphological changes were observed with extruded dioctadecyldimethylammonium chloride (DODAC) vesicles after dilution with a nonionic (sucrose) and ionic (CaCl₂) osmotic agentabove and below the gel-to-liquid crystalline transition temperature. By means of turbidimetry, dynamic light scattering,and cryo-transmission electron microscopy, it was seen that the vesicles only deflated when they were in the gel state,whereas in the liquid crystalline state, an ionic osmotic agent could induce twinning of the vesicles, reminiscent toendocytosis. The latter could occur as a result of the combined effects of reduced repulsion, local dehydration, andreduced bending rigidity induced by the ionic agent.