Pressurized Liquid Extraction and HPLC Quantification of Folic Acid in Fortified Wheat Flours
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- 作者：Michel Mozeika Ara煤jo ; Eric Marchioni ; Anna Lucia Casa帽as Haasis Villavicencio ; Minjie Zhao ; Pierre Zimmermann ; Etienne El-Khoury ; Martine Bergaentzle
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2012
- 出版时间：August 8, 2012
- 年：2012
- 卷：60
- 期：31
- 页码：7629-7633
- 全文大小：224K
- 年卷期：v.60,no.31(August 8, 2012)
- ISSN：1520-5118
文摘
A pressurized liquid extraction (PLE) method using phosphate buffer as solvent was applied for folic acid (FA) extraction from fortified wheat flours and was compared to a standard solid鈥搇iquid extraction (SLE) method. Extracted FA was quantified by reverse phase high-performance liquid chromatography (RP-HPLC) hyphenated with a phenyl column and an absorption photometric detector (位 = 280 nm). Detection and quantification limits were 0.12 and 0.4 ng, respectively, corresponding to 0.06 and 0.2 渭g g鈥? of analyzed wheat flour. Equivalent FA contents were found by both extraction methods, but a single PLE allowed a total recovery of FA content, whereas at least three successive SLEs were needed to achieve a total recovery of FA. The obtained results indicated that PLE is a rapid and efficient technique for FA extraction from fortified wheat flour.