Glutathione is a crucial component of the redox homeostasis of cells, and altered levels have been linked to human pathologies. We constructeda latent fluorophore (RhoSS) that responds to cellular thiols in vitro and in cyto following intracellular reduction by glutathione to yieldrhodamine 110. Importantly, RhoSS was demonstrated to respond to changing levels of glutathione in cells. This compound represents aclass of rationally designed latent fluorophores with exciting potential for monitoring cellular thiols.