Profiling and Quantification of Isoflavonoids in Kudzu Dietary Supplements by High-Performance Liquid Chromatography and Electrospray Ionization Tandem Mass Spectrometry

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• 作者：Jeevan K. Prasain ; Kenneth Jones ; Marion Kirk ; Landon Wilson ; Michelle Smith-Johnson ; Connie Weaver ; and Stephen Barnes
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2003
• 出版时间：July 16, 2003
• 年：2003
• 卷：51
• 期：15
• 页码：4213 - 4218
• 全文大小：134K
• 年卷期：v.51,no.15(July 16, 2003)
• ISSN：1520-5118

文摘

The kudzu vine (Pueraria sp.) is a rich source of isoflavones. Dietary supplements based on kudzuhave become commercially available. In the present study, liquid chromatography coupled withnegative and positive electrospray ionization tandem mass spectrometry (MS/MS) and diode arraydetection (DAD) has been used for the detection and characterization of isoflavonoids in kudzu dietarysupplements (KDS). The MS/MS spectrum of the protonated ion of puerarin showed characteristicproduct ions of the C-glycoside unit itself, whereas daidzin generated an abundant Y₀⁺ aglycon ionin its product ion spectrum. A base peak due to the loss of 120 Da [M + H - 120]⁺ is the diagnosticion for C-glycosides. Neutral loss scans allowed for the detection of other C- and O-glycosides in themethanolic extract of KDS, and their structures have been proposed. The concentration of isoflavonoidsin the methanolic extract of commercially available KDS was quantified by using DAD-HPLC. Puerarin,rather than daidzin, was the most abundant component (8.44-30.60 mg/capsule) in commerciallyavailable KDS.Keywords: Pueraria lobota; kudzu; isoflavonoids; C-glycosides; LC-MS/MS