Biocompatible Polylactide-block-Polypeptide-block-Polylactide Nanocarrier

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• 作者：Robert Dorresteijn ; Ruben Ragg ; Gianluca Rago ; Nils Billecke ; Mischa Bonn ; Sapun H. Parekh ; Glauco Battagliarin ; Kalina Peneva ; Manfred Wagner ; Markus Klapper ; Klaus M眉llen
• 刊名：Biomacromolecules
• 出版年：2013
• 出版时间：May 13, 2013
• 年：2013
• 卷：14
• 期：5
• 页码：1572-1577
• 全文大小：318K
• 年卷期：v.14,no.5(May 13, 2013)
• ISSN：1526-4602

文摘

Polypeptides are successfully incorporated into poly(l-lactide) (PLLA) chains in a ring-opening polymerization (ROP) of l-lactide by using them as initiators. The resulting ABA triblock copolymers possess molecular weights up to 11000 g路mol^鈥? and polydispersities as low as 1.13, indicating the living character of the polymerization process. In a nonaqueous emulsion, peptide-initiated polymerization of l-lactide leads to well-defined nanoparticles, consisting of PLLA-block-peptide-block-PLLA copolymer. These nanoparticles are easily loaded by dye-encapsulation and transferred into aqueous media without aggregation (average diameter of 100 nm) or significant dye leakage. Finally, internalization of PLLA-block-peptide-block-PLLA nanoparticles by HeLa cells is demonstrated by a combination of coherent anti-Stokes Raman spectroscopy (CARS) and fluorescence microscopy. This demonstrates the promise of their utilization as cargo delivery vehicles.