Individual Human Metabolic Phenotype Analyzed by 1H NMR of Saliva Samples

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• 作者：Sandra Wallner-Liebmann ; Leonardo Tenori ; Antonio Mazzoleni ; Martina Dieber-Rotheneder ; Manuela Konrad ; Peter Hofmann ; Claudio Luchinat ; Paola Turano ; Kurt Zatloukal
• 刊名：Journal of Proteome Research
• 出版年：2016
• 出版时间：June 3, 2016
• 年：2016
• 卷：15
• 期：6
• 页码：1787-1793
• 全文大小：298K
• 年卷期：0
• ISSN：1535-3907

文摘

Saliva is an important physiological fluid that contains a complex mixture of analytes that may produce a characteristic individual signature. In recent years, it has been demonstrated that urine possesses a clear signature of the individual metabolic phenotype. Here NMR-based metabolomics was employed to analyze saliva from 23 healthy volunteers. About six saliva samples were collected daily from each individual for 10 consecutive days: 7 days in a real-life situation (days 1–7, Phase I) and 3 days (days 8–10, Phase II) under a standardized diet plus a physical exercise program at day 10. The result is the first demonstration of the existence of an individual metabolic phenotype in saliva. A systematic comparative analysis with urine samples from the same collection scheme demonstrates that the individual phenotype in saliva is slightly weaker than that in urine but less influenced by diet.