Curvature Sensing by a Viral Scission Protein

详细信息    查看全文

• 作者：Agnieszka Martyna ; Jordi Gómez-Llobregat ; Martin Lindén ; Jeremy S. Rossman
• 刊名：Biochemistry
• 出版年：2016
• 出版时间：June 28, 2016
• 年：2016
• 卷：55
• 期：25
• 页码：3493-3496
• 全文大小：279K
• 年卷期：0
• ISSN：1520-4995

文摘

Membrane scission is the final step in all budding processes wherein a membrane neck is sufficiently constricted so as to allow for fission and the release of the budded particle. For influenza viruses, membrane scission is mediated by an amphipathic helix (AH) domain in the viral M2 protein. While it is known that the M2AH alters membrane curvature, it is not known how the protein is localized to the center neck of budding virions where it would be able to cause membrane scission. Here, we use molecular dynamics simulations on buckled lipid bilayers to show that the M2AH senses membrane curvature and preferentially localizes to regions of high membrane curvature, comparable to that seen at the center neck of budding influenza viruses. These results were then validated using in vitro binding assays to show that the M2AH senses membrane curvature by detecting lipid packing defects in the membrane. Our results show that the M2AH senses membrane curvature and suggest that the AH domain may localize the protein at the viral neck where it can then mediate membrane scission and the release of budding viruses.