Biflavonoids Isolated from Selaginella lepidophylla Inhibit Photosynthesis in Spinach Chloroplasts

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• 作者：Marí ; a Isabel Aguilar ; Maryosawa G. Romero ; Marí ; a Isabel Chá ; vez ; Beatriz King-Dí ; az ; Blas Lotina-Hennsen
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2008
• 出版时间：August 27, 2008
• 年：2008
• 卷：56
• 期：16
• 页码：6994-7000
• 全文大小：227K
• 年卷期：v.56,no.16(August 27, 2008)
• ISSN：1520-5118

文摘

Through bioactivity-guided chemical analysis of d-content content-type="genus-species" type="simple">Selaginella lepidophyllad-content>, biflavonoids robustaflavone (1), 2,3-dihydrorobustaflavone (2), and 2,3-dihydrorobustaflavone-5-methyl ether (3) were isolated and their structures confirmed by spectroscopic and spectrometric analyses. Their NMR resonances were unambiguously assigned from HMBC, NOESY, and NOESY-1D experiments, and absolute configurations of 2 and 3 were established. Compound 3 has not been reported, and although structure of 2 was described before, the ¹³C NMR assignment does not correlate with the structure reported. Therefore, this is the first report of 2. All compounds inhibited ATP production. Compounds 1 and 2 behaved as Hill reaction inhibitors. 1 interacted with photosystem II, transforming the reaction centers to silent centers at 300 and 600 μM. The interaction and inhibition target of 2 was located on Cyt b₆f to PC. The three compounds also behaved as energy transfer inhibitors, 3 being the most active.