Single-Molecule Fluorescence Photoswitching of a Diarylethene鈭扨erylenebisimide Dyad: Non-destructive Fluorescence Readout

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• 作者：Tuyoshi Fukaminato ; Takao Doi ; Nobuyuki Tamaoki ; Katsuki Okuno ; Yukihide Ishibashi ; Hiroshi Miyasaka ; Masahiro Irie
• 刊名：Journal of the American Chemical Society
• 出版年：2011
• 出版时间：April 6, 2011
• 年：2011
• 卷：133
• 期：13
• 页码：4984-4990
• 全文大小：901K
• 年卷期：v.133,no.13(April 6, 2011)
• ISSN：1520-5126

文摘

Single-molecule fluorescence photoswitching plays an essential role in ultrahigh-density (Tbits/inch²) optical memories and super-high-resolution fluorescence imaging. Although several fluorescent photochromic molecules and fluorescent proteins have been applied, so far, to optical memories and super-high-resolution imaging, their performance is unsatisfactory because of the absence of 鈥渘on-destructive fluorescence readout capability鈥? Here we report on a new molecular design principle of a molecule having non-destructive readout capability. The molecule is composed of acceptor photochromic diarylethene and donor fluorescent perylenebisimide units. The fluorescence is reversibly quenched when the diarylethene unit converts between the open- and the closed-ring isomers upon irradiation with visible and UV light. The fluorescence quenching is based on an electron transfer from the donor to the acceptor units. The fluorescence photoswitching and non-destructive readout capability were demonstrated in solution (an ensemble state) and at the single-molecule level. Femtosecond time-resolved transient and fluorescent lifetime measurements confirmed that the fluorescence quenching is attributed to the intramolecular electron transfer.