Hydroxyapatite Nanoparticles as Particulate Emulsifier: Fabrication of Hydroxyapatite-Coated Biodegradable Microspheres

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• 作者：Syuji Fujii ; Masahiro Okada ; Hidekatsu Sawa ; Tsutomu Furuzono ; Yoshinobu Nakamura
• 刊名：Langmuir
• 出版年：2009
• 出版时间：September 1, 2009
• 年：2009
• 卷：25
• 期：17
• 页码：9759-9766
• 全文大小：1066K
• 年卷期：v.25,no.17(September 1, 2009)
• ISSN：1520-5827

文摘

Hydroxyapatite (HAp) nanoparticle-coated micrometer-sized poly(l-lactic acid) (PLLA) microspheres were fabricated via a “Pickering-type” emulsion route in the absence of any molecular surfactants. Stable oil-in-water emulsions were prepared using 40 nm HAp nanoparticles as a particulate emulsifier and a dichloromethane (CH₂Cl₂) solution of PLLA as an oil phase. It was clarified that the interaction between carbonyl/carboxylic acid groups of PLLA and the HAp nanoparticles at the CH₂Cl₂−water interface played a crucial role to prepare the stable Pickering-type emulsion. The HAp nanoparticle-coated PLLA microspheres were fabricated by the evaporation of CH₂Cl₂ from the emulsion and characterized in terms of size, particle size distribution, and morphology using scanning/transmission electron microscopes. Scanning electron microscopy study and ultrathin cross section observation using transmission electron microscopy confirmed adsorption of the HAp nanoparticles only at the surface of the PLLA microspheres. Cell-adhesion experiments suggested the HAp nanoparticles on the surface of the PLLA microspheres promoted the cell adhesion and spreading.