文摘
While islet amyloid deposition comprising amylin is one of pathological hallmarks of type 2 diabetes mellitus (T2DM), no useful amylin-imaging probe has been reported. In this study, we evaluated two 99mTc-labeled pyridyl benzofuran derivatives as novel amylin-imaging probes using the newly established islet amyloid model mouse. Binding experiments in vitro demonstrated that [99mTc]1 displayed a higher affinity for amylin aggregates than [99mTc]2. Autoradiographic studies using human pancreas sections with T2DM revealed that [99mTc]1 clearly labeled islet amyloid in T2DM pancreatic sections, while [99mTc]2 did not. Although the initial uptake of [99mTc]1 by the normal mouse pancreas was low (0.74%ID/g at 2 min post-injection), [99mTc]1 showed higher retention in the model mouse pancreas than that of the normal mouse, and exhibited strong binding to amylin aggregates in the living pancreas of the model mice. These results suggest that [99mTc]1 is a potential imaging probe targeting islet amyloids in the T2DM pancreas.