Rapid Detection of a Cocaine-Binding Aptamer Using Biological Nanopores on a Chip

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• 作者：Ryuji Kawano ; Toshihisa Osaki ; Hirotaka Sasaki ; Masahiro Takinoue ; Satoko Yoshizawa ; Shoji Takeuchi
• 刊名：Journal of the American Chemical Society
• 出版年：2011
• 出版时间：June 8, 2011
• 年：2011
• 卷：133
• 期：22
• 页码：8474-8477
• 全文大小：770K
• 年卷期：v.133,no.22(June 8, 2011)
• ISSN：1520-5126

文摘

This paper describes a methodology for the rapid and highly selective detection of cocaine using a membrane protein channel combined with a DNA aptamer. The DNA aptamer recognizes the cocaine molecule with high selectivity. We successfully detected a low concentration of cocaine (300 ng/mL, the drug test cutoff limit) within 60 s using a biological nanopore embedded in a microchip.