文摘
1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity of the 70% aqueous acetone extractfrom the seed coat of the brown soybean variety, Akita-Zairai, was investigated. The activity of theseed coat of Akita-Zairai was much higher than that of three other reddish-brown varieties, butlower than that of two black varieties, and was closely dependent on the content of phenoliccompounds. In the LH20 column chromatography of Akita-Zairai, high DPPH radical-scavengingactivities were detected in the fractions eluted with MeOH and 70% aqueous acetone. Proanthocyanidins were also detected in fractions showing high radical-scavenging activities. Matrix-assistedlaser desorption/ionization-time-of-flight mass spectrometry analysis showed that the degree ofpolymerization (DP) of the procyanidins contained in the brown or black soybean seed coat was ashigh as DP30.Keywords: Soybean; Glycine max; procyanidin polymer; radical-scavenging activity; brown seedcoat; MALDI-TOFMS