Exploring the Active Site Structure of a Photoreceptor Protein by Raman Optical Activity

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• 作者：Masashi Unno ; Takashi Kikukawa ; Masato Kumauchi ; Naoki Kamo
• 刊名：The Journal of Physical Chemistry B
• 出版年：2013
• 出版时间：February 7, 2013
• 年：2013
• 卷：117
• 期：5
• 页码：1321-1325
• 全文大小：323K
• 年卷期：v.117,no.5(February 7, 2013)
• ISSN：1520-5207

文摘

We have developed a near-infrared excited Raman optical activity (ROA) spectrometer and report the first measurement of near-infrared ROA spectra of a light-driven proton pump, bacteriorhodopsin. Our results demonstrate that a near-infrared excitation enables us to measure the ROA spectra of the chromophore within a protein environment. Furthermore, the ROA spectra of the all-trans, 15-anti and 13-cis, 15-syn isomers differ significantly, indicating a high structural sensitivity of the ROA spectra. We therefore expect that future applications of the near-infrared ROA will allow the experimental elucidation of the active site structures in other proteins as well as reaction intermediates.