Reactive Microcontact Printing of DNA Probes on (DMA-NAS-MAPS) Copolymer-Coated Substrates for Efficient Hybridization Platforms
详细信息 查看全文
- 作者：Rossella Castagna ; Alessandro Bertucci ; Eko Adi Prasetyanto ; Marco Monticelli ; Dario Valter Conca ; Matteo Massetti ; Parikshit Pratim Sharma ; Francesco Damin ; Marcella Chiari ; Luisa De Cola ; Riccardo Bertacco
- 刊名：Langmuir
- 出版年：2016
- 出版时间：April 5, 2016
- 年：2016
- 卷：32
- 期：13
- 页码：3308-3313
- 全文大小：347K
- ISSN：1520-5827
文摘
High-performing hybridization platforms fabricated by reactive microcontact printing of DNA probes are presented. Multishaped PDMS molds are used to covalently bind oligonucleotides over a functional copolymer (DMA-NAS-MAPS) surface. Printed structures with minimum width of about 1.5 μm, spaced by 10 μm, are demonstrated, with edge corrugation lower than 300 nm. The quantification of the immobilized surface probes via fluorescence imaging gives a remarkable concentration of 3.3 × 103 oligonucleotides/μm2, almost totally active when used as probes in DNA-DNA hybridization assays. Indeed, fluorescence and atomic force microscopy show a 95% efficiency in target binding and uniform DNA hybridization over printed areas.