Polyaniline Langmuir-Blodgett Film Based Cholesterol Biosensor
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- 作者：Zimple Matharu ; G. Sumana ; Sunil K. Arya ; S. P. Singh ; Vinay Gupta ; B. D. Malhotra
- 刊名：Langmuir
- 出版年：2007
- 出版时间：December 18, 2007
- 年：2007
- 卷：23
- 期：26
- 页码：13188 - 13192
- 全文大小：146K
- 年卷期：v.23,no.26(December 18, 2007)
- ISSN：1520-5827
文摘
Cholesterol oxidase (ChOx) has been covalently linked to Langmuir-Blodgett (LB) monolayers of polyaniline(PANI)-stearic acid (SA) prepared onto indium-tin-oxide (ITO) coated glass plates via glutaraldehyde (Glu) chemistry.These ChOx/Glu/PANI-SA LB film/ITO electrodes have been characterized by FT-IR, cyclic voltammetry, andscanning electron microscopy, respectively. The results of response measurements carried out on these bioelectrodesusing linear sweep voltammetry (LSV) reveal linearity from 25 to 400 mg/dL of cholesterol concentration withsensitivity of 88.9 nA mg-1 dL. The linear regression analysis of bioelectrode reveals standard deviation and correlationcoefficient of 0.737 A and 0.9988, respectively. The low value of the Michaelis-Menten constant of these bioelectrodesobtained as 1.21 mM for the immobilized enzyme indicates increased interaction between ChOx and cholesterol inthe PANI-SA LB film.